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重點項目

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產品簡介:

       外顯子組是指全部外顯子區域的集合,該區域包含合成蛋白質所需要的重要信息,涵蓋了與個體表型相關的大部分功能性變異。全外顯子組分捕獲析是指利用序列捕獲技術將全基因組外顯子區域DNA捕捉并富集后進行高通量分析的基因組分析方法。外顯子組序列捕獲高通量分析可以高特異性和高覆蓋率捕獲研究者感興趣的目標外顯子區域,可用于尋找復雜疾病(如:癌癥、糖尿病、肥胖癥等)的致病基因和易感基因等相關研究。

 


 
         我們接受DNA樣本或已構建好的全外顯子組DNA文庫,并回郵捕獲好的全外顯子DNA或數據。同時我們也可以單獨提供捕獲試劑盒,以滿足您的需要。公司目前擁有成熟的人類(52M),山羊(30M)等物種全外顯子捕獲試劑盒等。

實驗流程:
 
技術優勢:

         全外顯子檢測是一種新型的基因組分析方法,指利用序列捕獲技術將全基因組外顯子區域DNA捕捉并富集后進行高通量測序,可以一次性對人類基因組中的全部外顯子進行測序分析,從而找出常見變異、罕見變異、以及發現新基因。

 
全外顯子的技術優勢:

1. 直接對蛋白編碼序列進行測序,可以找出影響蛋白結構的變異。
2. 高深度測序,檢測靈敏度達2%,即可檢測頻率低于2%的罕見變異 。
3. 全外顯子區域約占人類全部基因序列中最關鍵的1-2%,能夠有效降低費用、周期和工作量,是探索遺傳疾病致病基因的最常見方法。
4、 我們接受DNA樣本或已構建好的全外顯子組DNA文庫,并回郵捕獲好的全外顯子DNA或數據。同時我們也可以單獨提供捕獲試劑盒,以滿足您的需要。公司目前擁有成熟的人類(52M),山羊(30M)等物種全外顯子捕獲試劑盒等。

 


 

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